comparison of survival and developmental rates of mouse ovarian follicles after two and three dimensional cultures

objective: in vitro ovarian follicle culture provides a tool to investigate folliculogenesis and may be used as an assisted reproductive technology )art(. this study aims to compare survival and development rates in mouse ovarian follicles after two and three dimensional in vitro cultures. methods: preantral follicles were isolated from the ovaries of 14-day old female mice and cultured in α-mem medium supplemented with 5% fbs for 12 days in a two dimensional and three dimensional culture with different concentrations of sodium alginate (0.25%, 0. 5%, or 1%). the follicle diameter, survival and maturation rate during culture were analyzed and compared with one-way analysis of variance (anova). p